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How to demultiplex a count matrix and convert it to Cellenics®-compatible 10X files
A brief tutorial written in R to demultiplex an rds object and convert it to 10x files (count matrices), which can be directly uploaded to Cellenics®.
RNA van Winkle writes...
Steven Salvini completes his first single cell RNA sequencing (scRNA-seq) analysis by using Cellenics to reproduce results from Phoebe Kirkwood’s (2021) paper.
Top 5 Most Revolutionary Uses of Single-Cell RNA Sequencing
The use of single-cell RNA sequencing (scRNA-seq) in biomedical research has skyrocketed in recent years. The reason for the growing popularity of this technology is that scRNA-seq enables researchers to understand the cellular heterogeneity and functional diversity in a sample . This has led to new and ground-breaking discoveries in several fields, including development, oncology, immunology, neuroscience, and the study of rare diseases. In this blog post, we take a detailed look at some of these recent breakthroughs through the lens of the main scRNAseq applications!
How scRNA-seq Became the New Hot Topic?
Despite their apparent similarities, cells can show unique characteristics when examined closely. A cell’s ability to function within the body depends on the right genes being switched on at the right time. Gene expression also varies according to the cell type and developmental stage. Looking at these details has been made possible with single-cell RNA sequencing!